The effect of a preparation containing glycocholic acid on the biocidal efficacy of sodium hypochlorite in a biofilm laboratory model

doi:10.1111/aej.12771

Q3 · DentistryImpact Factor · 1.202

AutoresYoseif Haddad, Wing Chuan Chan, William Nguyen Ha, Antonio Conde, Roberto Estévez, Alejandro Peña López, Georgia Charlotte Gates, Giampiero Rossi‐Fedele

Por qué importa

Este estudio en un modelo de biofilm de conducto radicular evalúa la eficacia biocida del hipoclorito de sodio (NaOCl) al 1% y 4%, tanto solo como mezclado con un preparado que contiene glicocolato. El objetivo era determinar si la adición de este preparado, denominado Keratobacter (KB), podía mejorar la acción desinfectante del NaOCl en condiciones de laboratorio que simulan un ambiente infectado. Los resultados indican que la adición de KB no mejoró significativamente la capacidad del NaOCl al 4% para reducir las unidades formadoras de colonias bacterianas, aunque el NaOCl al 4% por sí solo fue el único capaz de obtener cultivos negativos en todas las muestras. Estos hallazgos sugieren que, en este modelo específico, la combinación con KB no aporta beneficios claros sobre la eficacia desinfectante del NaOCl en endodoncia.

Abstract original

This study assessed the antimicrobial effect of sodium hypochlorite (NaOCl) mixtures combined with Keratobacter (KB) using an engineered biofilm root canal model. Clinical and reagent grade NaOCl were mixed with KB (9:1-vol/vol) to assess pH values over 1 min to select the ideal solution with a pH just below the pKa of hypochlorous acid. The samples were randomly divided into five groups: 1% and 4% NaOCl reagents, a mixture of NaOCl:KB using 1% and 4% NaOCl reagents and distilled water. Outcome measures were colony-forming units (CFUs/mL) and positive/negative cultures. No significant differences were observed in the pairwise comparisons between 1%, 4% NaOCl and 4% NaOCl+KB for the outcome CFUs/mL. Only 4% NaOCl presented with negative cultures in all samples, whereas 1% NaOCl and 4% NaOCl+KB had similar results (54% vs. 40%). The addition of KB has a limited effect on the antimicrobial efficacy of 4% NaOCl in this laboratory model.

Leer texto completo ↗DOI · 10.1111/aej.12771

Autor Doctor&Cols

Alejandro Peña López
Endodoncia y Cirugía Apical

Cómo citar

Haddad Y, Chan WC, Ha WN, Conde A, Estévez R, López AP, et al. The effect of a preparation containing glycocholic acid on the biocidal efficacy of sodium hypochlorite in a biofilm laboratory model. Australian Endodontic Journal. 2023. doi:10.1111/aej.12771