Sodium Hypochlorite and a Preparation Containing Glycocholic Acid and Surfactants Have a Synergistic Action on Organic Tissue Dissolution In Vitro
★ Alto impactoQ1 · DentistryImpact Factor · 3.424
AutoresAlejandro Peña López, Antonio Conde, Roberto Estévez, Óliver Valencia de Pablo, Giampiero Rossi‐Fedele, Rafael Cisneros
Por qué importa
Este estudio in vitro evalúa la acción sinérgica del hipoclorito de sodio (NaOCl) con un preparado coadyuvante que contiene ácido glicocólico y tensioactivos (Keratobacter, KB) sobre la disolución de tejido orgánico, utilizando mucosa de paladar de cerdo como modelo. Se observó que la combinación de NaOCl y KB incrementó significativamente la reducción del peso de las muestras en comparación con cada agente por separado, especialmente en los primeros minutos de exposición. Aunque la disolución completa no se alcanzó, estos hallazgos sugieren que la adición de KB al NaOCl podría potenciar la eficacia de la irrigación en la limpieza de conductos radiculares, facilitando la eliminación de tejido orgánico residual. Estos resultados son relevantes para optimizar los protocolos de irrigación en endodoncia, mejorando la limpieza y desinfección de los sistemas de conductos.
The aim of this study was to evaluate porcine palatal mucosa dissolution by sodium hypochlorite (NaOCl) with or without an auxiliary dissolving agent containing glycocholic acid and a mixture of surfactants (Keratobacter [KB]; Saint Joseph DID, Valencia, Spain). One hundred forty samples were obtained from porcine palatal mucosa and weighed using a high-precision balance. The samples were randomly divided into 4 experimental groups (n = 35) based on the test solution used: distilled water (the negative control), CanalPro NaOCl 6% (Coltene Whaledent, Altstätten, Switzerland), KB, and a 9:1 vol/vol mixture of NaOCl with KB (NaOCl + KB). After 5, 10, 15, and 20 minutes of immersion in the solutions at 27°C ± 1°C, the samples were weighted by a blinded assessor. The intergroup weight at the different time points was statistically analyzed using the analysis of variance test with the Bonferroni posttest. All test groups presented with tissue dissolution although complete dissolution did not occur in any sample. The largest percent in weight reduction occurred between time points (t) = 0 minutes and t = 5 minutes for the NaOCl + KB group (22.5%) followed by KB (18.5%) for the same time period. NaOCl presented with similar tissue dissolution activity during the different time points, ranging from 7.8% (t = 10 minutes-t = 15 minutes) to 6.8% (t = 15 minutes-t = 20 minutes). Significant weight differences were found among the different experimental groups after 5, 10, and 15 minutes of incubation, with the only exception being KB versus NaOCl + KB. No significant differences were found when comparing the test groups at t = 20 minutes. The addition of KB to NaOCl increased porcine palatal mucosa dissolution in vitro.
López AP, Conde A, Estévez R, Pablo ÓVD, Rossi‐Fedele G, Cisneros R. Sodium Hypochlorite and a Preparation Containing Glycocholic Acid and Surfactants Have a Synergistic Action on Organic Tissue Dissolution In Vitro. Journal of Endodontics. 2018. doi:10.1016/j.joen.2018.01.007
