Sinus floor elevation using particulate PLGA‐coated biphasic calcium phosphate bone graft substitutes: A prospective histological and radiological study

doi:10.1111/cid.12741

AutoresAntonio Juan Flichy‐Fernández, Juan Antonio Blaya-Tárraga, Francisco O’Valle, Miguel Padial‐Molina, Miguel Peñarrocha Diago, Pablo Galindo‐Moreno

Por qué importa

Este estudio prospectivo investiga la eficacia de biomateriales de fosfato de calcio bifásico recubiertos de PLGA en la elevación del seno maxilar, comparándolos con el material no recubierto. Aunque no se hallaron diferencias radiológicas ni histomorfométricas significativas en la ganancia ósea, el análisis inmunohistoquímico reveló que las partículas recubiertas de PLGA mostraron una mayor colonización por precursores osteoblásticos positivos para Musashi-1 y un mayor desarrollo de estructuras vasculares. Estos hallazgos sugieren que el recubrimiento de PLGA podría potenciar la respuesta celular y la vascularización en el sitio de regeneración ósea, un aspecto relevante para la futura integración de implantes en pacientes que requieren este tipo de procedimientos pre-protésicos.

Abstract original

BACKGROUND: Poly (lactic-co-glycolic acid) (PLGA) is widely used for the development of delivery systems for drugs and therapeutic biomolecules in tissue engineering applications. Particles of biphasic calcium phosphate can be covered by PLGA to change their manipulating characteristics. PURPOSE: Aim of this study was to investigate the radiological and histomorphometric results of the use of PLGA-coated biphasic calcium phosphate granules in sinus floor elevation and to analyze the underlying molecular processes by immunohistochemical staining. MATERIALS AND METHODS: A randomized clinical study was designed to include patients in need of sinus floor elevation. Patients were assigned to receive either PLGA-coated biphasic calcium phosphate particles (group I) or the equivalent but noncoated particles (group II). Cone beam computed tomography (CBCT) scans were performed before and 6 months after the procedure to assess the bone height gain. At the time of implant placement, bone core biopsies were obtained at the site of implant placement. Histological sections were subjected to histomorphometric and immunohistochemical evaluation of differentiation markers (Musashi-1 [MSI1]). RESULTS: No statistically significant differences were observed between groups for the radiologic parameters. No differences were observed histologically or histomorphometrically. However, PLGA-coated particles (group I) were more colonized by MSI1-positive osteoblast precursors (P = 0.0001, chi-squared test) and were penetrated by more CD34-positive vascular structures (P = 0.001, chi-squared test) than noncoated particles (group II). CONCLUSIONS: PLGA-coated particles are associated with more MSI11-positive cells and more extensive microvascularization than noncoated particles.

Leer texto completo ↗DOI · 10.1111/cid.12741

Autor Doctor&Cols

Toni Flichy
Cirugía Oral e Implantología

Cómo citar

Flichy‐Fernández AJ, Blaya-Tárraga JA, O’Valle F, Padial‐Molina M, Diago MP, Galindo‐Moreno P. Sinus floor elevation using particulate PLGA‐coated biphasic calcium phosphate bone graft substitutes: A prospective histological and radiological study. Clinical Implant Dentistry and Related Research. 2019. doi:10.1111/cid.12741